Recombinant Mouse Interferon Gamma Receptor 1, Fc-tagged

• Cat.No.: Ifngr1-1748M
• Product Overview: Recombinant Mouse Ifngr1 encoding the extracellular domain was fused to the carboxy-terminal Fc region of human IgG1 via a linker peptide, inserted into a suitable mammalian expression vector and expressed inNSO cells.

Specification

• Cat. No.: Ifngr1-1748M
• Description: IFN-γ receptor ligand-binding chain (IFNγR1) or IFN-γ R α chain, also known CDw119, is member of the class II cytokine receptor family. The IFN-γ receptor is a complex of a high affinity IFN-γ-binding chain (aka, IFN-γ R α chain) and a second accessory protein required for signal transduction known as IFN-γ R β chain. Binding of IFN-γ induces receptor dimerization and internalization. Signal transduction involves Jak1 and Jak2 protein kinases and involves STAT1 activation. The IFN-γ receptor is expressed at moderate levels on virtually every cell with the exception of erythrocytes.
• Molecular Weight: The predicted molecular weight of Recombinant Mouse IFN-γ R1 is Mr 52 kDa. However, the actual molecular weight as observed by migration on SDS Page is Mr 70-90 kDa.
• Source: Mammalian cells
• State Of Matter: Lyophilized.
• Purity: >95% by SDS Page and analyzed by silver stain.
• Endotoxin: <1.0 EU/µg as determined by the LAL method.
• Biological Activity: The biological activity of Mouse IFN-γ R1 was determined by its ability to inhibit rmIFN-γ mediated protection of mouse L929 cells viral lysis. The expected ED50 is typically 0.4 - 2 μg/mL in the presence of 1 ng/mL of rmIFN-γ.
• Storage And Stability: This lyophilized protein is stable for six to twelve months when stored desiccated at -20°Cto -70°C. After aseptic reconstitution, this protein may be stored at 2°Cto 8°Cfor one month or at -20°Cto -70°Cin a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions.

Gene Information

• Gene Name: Ifngr1 interferon gamma receptor 1 [ Mus musculus ]
• Synonyms: Ifngr1; interferon gamma receptor 1; Ifgr; CD119; Ifngr; Nktar; IFN-gammaR; IFN-gamma R; INF-g receptor
• Gene ID: 15979
• mRNA Refseq: NM_010511
• Protein Refseq: NP_034641
• UniProt ID: P15261
• Chromosome Location: 10 A3; 10 15.0 cM
• Pathway: Cytokine-cytokine receptor interaction; Jak-STAT signaling pathway; Natural killer cell mediated cytotoxicity
• Function: cytokine binding; receptor activity