Recombinant Mouse IL12A & IL12B Overexpression Lysate(Met 1-Ala 215&Met 1-Ser 335)

• Cat.No.: IL12A & IL12B-1780MCL
• Product Overview: Recombinant Mouse IL12A (P43431) (Met 1-Ala 215) was fused with a polyhistidine tag at the C-terminus, constructed the plasmid 1; Recombinant Mouse (P43432) (Met 1-Ser 335) was fused with a polyhistidine tag at the C-terminus, constructed the plasmid 2. The two plasmids were co-expressed and the heterodimer was purified in HEK293.

Specification

• Species: Mouse
• Source: HEK293
• Tag: His
• Protein Length: Met 1-Ala 215&Met 1-Ser 335
• Form: Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
• Molecular Mass: The recombinant mouse IL12 heterodimer of IL12A/IL12B, comprises 528 (204 + 324) amino acids and has a calculated molecular mass of 60.3 (23.1 + 37.2) kDa. The apparent molecular mass of rh IL12 heterodimer is approximately 28 & 47 kDa respectively in SDS-PAGE under reducing conditions.
• Storage: Store at 4°C for up to twelve months from date of receipt. After re-dissolution, aliquot and store at -80°C for up to twelve months. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: Il12a interleukin 12a [ Mus musculus ]
• Official Symbol: IL12A
• Synonyms: IL12A; interleukin 12a; interleukin-12 subunit alpha; CLMF p35; IL-12 subunit p35; interleukin 12a p35 subunit; cytotoxic lymphocyte maturation factor 35 kDa subunit; p35; Ll12a; Il-12a; IL-12p35; MGC151228; MGC151232
• Gene ID: 16159
• mRNA Refseq: NM_001159424
• Protein Refseq: NP_001152896