Recombinant Mouse Igfbp2 Protein, Full Length, C-His tagged

• Cat.No.: Igfbp2-01MFL
• Product Overview: Recombinant mouse IGFBP-2 (Full length), fused to His-tag at C-terminus, was expressed in HEK293 cell and purified by using conventional chromatography techniques.

Specification

• Species: Mouse
• Source: HEK293
• Tag: His
• Protein Length: Full Length
• Description: IGFBP-2, also known as insulin-like growth factor binding protein 2, is a member of IGFBP superfamily. IGFBPs modulate the biological activities of IGF proteins and have a high affinity for IGFs. IGFBPs are not merely carrier proteins for IGFs, but hold a central position in IGF ligand-receptor interactions through influences on both the bioavailability and distribution of IGFs in the extracellular environment. During development, IGFBP-2 is expressed in a number of tissues. The highest expression level is found in the central nervous system. In adults, high expression levels are also detected in the central nervous system and in a number of reproductive tissues.This protein binds preferentially to IGF-2, exhibiting a 2 - 10 fold higher affinity for IGF-2 than for IGF-1. Also, it is overexpressed in many malignancies and is often correlated with an increasingly malignant status of the tumor, pointing to the potential involvement of IGFBP-2 in tumorigenesis.
• Form: Liquid
• Bio-activity: Measured by its ability to inhibit proliferation using MCF-7 human breast cancer cells in the presence of Mouse IGF-2. The ED50 range ≤ 0.7 μg/mL.
• Molecular Mass: 30.3 kDa (277aa)
• AA Sequence: EVLFRCPPCTPERLAACGPPPDAPCAELVREPGCGCCSVCARQEGEACGVYIPRCAQTLRCYPNPGSELPLKALVTGAGTCEKRRVGTTPQQVADSDDDHSEGGLVENHVDGTMNMLGGGSSAGRKPLKSGMKELAVFREKVNEQHRQMGKGAKHLSLEEPKKLRPPPARTPCQQELDQVLERISTMRLPDDRGPLEHLYSLHIPNCDKHGRYNLKQCKMSLNGQRGECWCVNPNTGKPIQGAPTIRGDPECHLFYNEQQETGGAHAQSVQ
• Endotoxin: < 1 EU/μg of protein (determined by LAL method)
• Purity: > 85% by SDS-PAGE
• Applications: SDS-PAGE, Bioactivity
• Notes: For research use only. This product is not intended or approved for human, diagnostics or veterinary use.
• Storage: Can be stored at +2 to +8 centigrade for 1 week. For long term storage, aliquot and store at -20 to -80 centigrade. Avoid repeated freezing and thawing cycles.
• Concentration: 1 mg/mL (determined by Absorbance at 280nm)
• Storage Buffer: Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol
• References: 1. Yoshiyuki H., et al, (1994) Cellular Physiology. 158:444-450.
  2. Agarwal N., et al, (1991) Exp Eye Res. 52:549-561.

Gene Information

• Gene Name: Igfbp2 insulin-like growth factor binding protein 2 [ Mus musculus (house mouse) ]
• Official Symbol: Igfbp2
• Synonyms: IGFBP2; insulin-like growth factor binding protein 2; insulin-like growth factor-binding protein 2; IGF-binding protein 2; IBP-2; Igfbp-2; AI255832; mIGFBP-2
• Gene ID: 16008
• mRNA Refseq: NM_008342
• Protein Refseq: NP_032368
• UniProt ID: P47877