Recombinant Mouse Icosl Protein, His-tagged, Alexa Fluor 647 conjugated

• Cat.No.: Icosl-595MAF647
• Product Overview: Alexa Fluor 647 conjugated recombinant Mouse Icosl (Glu47-Lys279) protein was fused to His-tag at C-terminus and expressed in human 293 cells (HEK293).

Specification

• Description: ICOS ligand (ICOSLG) is also known as B7 homolog 2 (B7-H2), B7-related protein 1 (B7RP-1) and CD antigen CD275, which belongs to the immunoglobulin superfamily and BTN/MOG family. ICOSLG contains one Ig-like C2-type (immunoglobulin-like) domain and one Ig-like V-type (immunoglobulin-like) domain. Isoform 1 is widely expressed, while isoform 2 is detected only in lymph nodes, leukocytes and spleen. B7-H2 is ligand for the T-cell-specific cell surface receptor ICOS. B7-H2 acts as a costimulatory signal for T-cell proliferation and cytokine secretion and induces also B-cell proliferation and differentiation into plasma cells. B7-H2 could play an important role in mediating local tissue responses to inflammatory conditions, as well as in modulating the secondary immune response by co-stimulating memory T-cell function.
• Source: HEK293
• Species: Mouse
• Tag: His
• Form: Lyophilized
• Molecular Mass: The protein has a calculated MW of 28.2 kDa. The protein migrates as 45-55 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Protein length: Glu47-Lys279
• N-terminal Sequence Analysis: Glu 47
• Endotoxin: < 1.0 EU/ μg by the LAL method.
• Purity: > 90 % as determined by SDS-PAGE
• Characteristic: Disulfide-linked homodimer
  Labeled with Alexa Fluor 647 via amines
  Excitation = 650 nm
  Emission = 668 nm
• Storage: For long term storage, the product should be stored at lyophilized state at -20 centigrade or lower.
  Please avoid repeated freeze-thaw cycles.
  This product is stable after storage at:
  -20 to -70 centigrade for 12 months in lyophilized state;
  -70 centigrade for 3 months under sterile conditions after reconstitution.
• Storage Buffer: Lyophilized from 0.22 μm filtered solution in PBS, pH7.4, 10% trehalose.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 μg/μL. Centrifuge the vial at 4 centigrade before opening to recover the entire contents.

Gene Information

• Gene Name: Icosl
• Official Symbol: Icosl
• Synonyms: ICOSL; icos ligand; ICOS ligand; B7 homolog 2; B7-like protein Gl50; B7-related protein 1; B7h; GI50; GL50; B7-H2; LICOS; B7RP-1; GL50-B; ICOS-L; Icoslg; Ly115l; AU044799; BG071784; mKIAA0653; KIAA0653
• Gene ID: 50723
• mRNA Refseq: NM_015790
• Protein Refseq: NP_056605
• UniProt ID: Q9JHJ8