Recombinant Mouse Htt protein, His & GST-tagged

• Cat.No.: Htt-1597M
• Product Overview: Recombinant Mouse Htt aa. (Thr782~Phe920) fused with N-terminal His & GST tag was produced in E. coli cells.

Specification

• Source: E. coli
• Species: Mouse
• Tag: His&GST
• Form: Freeze-dried powder
• Molecular Mass: 46kDa as determined by SDS-PAGE reducing conditions.
• Protein length: Thr782~Phe920
• Endotoxin: <1.0EU per 1ug (determined by the LAL method)
• Purity: >92%
• Applications: SDS-PAGE; WB; ELISA; IP; CoIP; Purification; Amine Reactive Labeling.
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
• Concentration: 200μg/mL
• Storage buffer: 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5%Trehalose and Proclin300.
• Reconstitution: Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
• Isoelectric Point: 6.5

Gene Information

• Gene Name: Htt huntingtin [ Mus musculus (house mouse) ]
• Official Symbol: Htt
• Synonyms: Htt; huntingtin; Hd; Hdh; IT15; AI256365; C430023I11Rik; HD protein homolog; Huntington disease gene homolog; huntington disease protein homolog
• Gene ID: 15194
• mRNA Refseq: NM_010414.3
• Protein Refseq: NP_034544.1
• UniProt ID: G3X9H5