Recombinant Mouse Hsd11b1 Protein, His-tagged

• Cat.No.: Hsd11b1-1636M
• Product Overview: Recombinant Mouse Hsd11b1 Protein (Met2-Asn292) with N-His tag was expressed in E. coli.

Specification

• Description: Enables 11-beta-hydroxysteroid dehydrogenase (NADP+) activity. Acts upstream of or within lung development. Predicted to be located in apical part of cell; nuclear membrane; and rough endoplasmic reticulum. Predicted to be integral component of endoplasmic reticulum membrane. Is expressed in several structures, including alimentary system; cardiovascular system; genitourinary system; nervous system; and sensory organ. Used to study cortisone reductase deficiency 2. Human ortholog(s) of this gene implicated in cortisone reductase deficiency; cortisone reductase deficiency 2; and obesity. Orthologous to human HSD11B1 (hydroxysteroid 11-beta dehydrogenase 1).
• Source: E. coli
• Species: Mouse
• Tag: His
• Form: Freeze-dried powder
• Molecular Mass: Predicted Molecular Mass: 36.0 kDa
  Accurate Molecular Mass: 34 kDa
• Protein length: Met2-Asn292
• Purity: > 90%
• Applications: Positive Control; Immunogen; SDS-PAGE; WB.
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 centigrade for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 centigrade for one month. Aliquot and store at -80 centigrade for 12 months.
• Storage Buffer: 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
• Reconstitution: Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Gene Information

• Gene Name: Hsd11b1 hydroxysteroid 11-beta dehydrogenase 1 [ Mus musculus (house mouse) ]
• Official Symbol: Hsd11b1
• Synonyms: HSD11B1; hydroxysteroid 11-beta dehydrogenase 1; corticosteroid 11-beta-dehydrogenase isozyme 1; 11-DH; 11-beta-HSD1; 11beta-HSD-1; 11beta-HSD1A; 11-beta-hydroxysteroid dehydrogenase 1; 11beta-hydroxysteroid dehydrogenase type 1; Corticosteroid 11-beta-dehydrogenase, isozyme 1 (11-DH) (11-beta-hydroxysteroid dehydrogenase 1) (11-beta-HSD1) (11beta-HSD1A)
• Gene ID: 15483
• mRNA Refseq: NM_001044751
• Protein Refseq: NP_001038216
• UniProt ID: P50172