Recombinant Mouse Hp protein, His-tagged

• Cat.No.: Hp-7754M
• Product Overview: Recombinant Mouse Hp aa. (Ala118~Cys281 (Accession # Q61646)) fused with N-terminal His tag was produced in E. coli cells.

Specification

• Description: This gene encodes a plasma glycoprotein called haptoglobin that binds free hemoglobin. The encoded preproprotein undergoes proteolytic processing to generate alpha and beta subunits that form a disulfide-linked tetrameric protein that plays an important role in the sequestration and clearance of extracorpuscular hemoglobin. Mice lacking the encoded protein exhibit stunted development of lymphoid organs associated with lower counts of mature T and B cells in the blood and secondary lymphoid compartments.
• Source: E. coli
• Species: Mouse
• Tag: His
• Form: Freeze-dried powder
• Molecular Mass: Predicted Molecular Mass: 19.8kDa
• Protein length: Ala118~Cys281
• Endotoxin: <1.0EU per 1µg (determined by the LAL method)
• Purity: >95%
• Characteristic: The isoelectric point is 9.
• Applications: SDS-PAGE; WB; ELISA; IP.
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
• Storage buffer: Supplied as lyophilized form in 20mM Tris, 500mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5% trehalose, and preservative.
• Reconstitution: Reconstitute in ddH2O.

Gene Information

• Gene Name: Hp haptoglobin [ Mus musculus (house mouse) ]
• Official Symbol: Hp
• Synonyms: HP-1; preHP2; zonulin; haptoglobin
• Gene ID: 15439
• mRNA Refseq: NM_001329965.1
• Protein Refseq: NP_001316894.1
• UniProt ID: Q61646

Reviews

07/02/2022

Its superior purity and stability ensure reliable and reproducible results, providing me with confidence in the integrity of my experiments.

01/18/2020

Its impeccable purity and stability ensure accurate and reproducible results, giving me confidence in the reliability of my research outcomes

07/07/2019

Whether I am investigating protein-protein interactions, studying enzymatic activity, or conducting protein structure analyses, the HP protein consistently delivers exceptional performance and accurate results.

Q&As

Can Haptoglobin levels be influenced by genetic factors?

Yes, genetic variations can affect Haptoglobin levels, and understanding these variations can be crucial for personalized medicine approaches.

How does Haptoglobin contribute to the body's defense against oxidative stress?

Haptoglobin acts as an antioxidant by binding to free hemoglobin, preventing the formation of reactive oxygen species that contribute to oxidative stress.

Are there any specific diseases where monitoring Haptoglobin is particularly crucial?

Conditions such as hemolytic anemias, infections, and chronic inflammatory diseases often require monitoring Haptoglobin levels for diagnostic and prognostic purposes.

How is the Haptoglobin test performed in a clinical setting?

The Haptoglobin test is usually conducted through a blood sample, and the serum or plasma is analyzed for Haptoglobin levels.

Can Haptoglobin be a diagnostic marker for conditions other than hemolysis and inflammation?

Yes, altered levels of Haptoglobin are associated with various conditions, including cardiovascular diseases, infections, and certain cancers.