Recombinant Mouse Hemolytic Complement, His-tagged

• Cat.No.: Hc-4909M
• Product Overview: Recombinant the 9 kDa mouse Hc protein expressed in E. coli contains a N-terminal His-Tag sequence.

Specification

• Species: Mouse
• Source: E.coli
• Tag: Non
• Description: Mouse Hc is a 77-amino acid peptide generated during complement activation from the α-chain of complement C5. Mouse Hc shares 60% and 82% amino acid sequence identity to human and rat Hc, respectively. Hc binds to a signaling G-protein coupled receptor (GPCR) (C5aR/CD88), inducing neutrophil chemotaxis and endothelial cell activation. It also triggers an oxidative burst in macrophages and neutrophils, and induces release of histamine in basophils and mast cells.
• Biological Activity: ED₅₀ 1-3 nM as determined by enzyme release assay of myeloperoxidase.
• Molecular Weight: 9 kDa
• Form: Lyophilized without additives.
• Appearance: Lyophilized protein
• Endotoxin Level: < 0.1 ng/μg
• Reconstitution: Reconstitute to a concentration of 1.0 mg/ml in ice-cold water containing 2.5 mg/ml BSA. The solution should be aliquoted and immediately stored at -70°C.
• Purity: >95% by SDS-PAGE
• Storage: Store at -20°C or -70°C as supplied. Stable for at least 1 year. After reconstition, store at -70°C.
• Pathways: Activation of C3 and C5; Complement Activation; Complement and Coagulation Cascades; Complement cascade; G alpha (i) signalling events; GPCR downstream signaling; GPCR ligand binding; Herpes simplex infection; Immune System; Innate Immunity Signaling; Peptide ligand-binding receptors; Pertussis; Prion diseases; Signaling by GPCR; Staphylococcus aureus infection

Gene Information

• Gene Name: Hc hemolytic complement [ Mus musculus ]
• Official Symbol: Hc
• Synonyms: Hc; hemolytic complement; C5; He; C5a; complement C5; OTTMUSP00000013195
• Gene ID: 15139
• mRNA Refseq: NM_010406
• Protein Refseq: NP_034536
• UniProt ID: P06684
• Chromosome Location: 2 cen-C1; 2 23.5 cM
• Function: C5a anaphylatoxin chemotactic receptor binding; endopeptidase inhibitor activity