Recombinant Mouse Grpr protein, His & GST-tagged

• Cat.No.: Grpr-7836M
• Product Overview: Recombinant Mouse Grpr aa. (Met1~Leu116) fused with N-terminal His & GST tag was produced in E. coli cells.

Specification

• Description: This gene encodes a multipass membrane protein that functions as a receptor for gastrin releasing peptide. Signalling through this receptor is important in a variety of physiological processes, including responses to itching, satiety, and fear.
• Source: E. coli
• Species: Mouse
• Tag: His&GST
• Form: Freeze-dried powder
• Molecular Mass: 43kDa as determined by SDS-PAGE reducing conditions.
• Protein length: Met1~Leu116
• Endotoxin: <1.0EU per 1ug (determined by the LAL method)
• Purity: >98%
• Characteristic: The isoelectric point is 7.6.
• Applications: Positive Control; Immunogen; SDS-PAGE; WB.
• Stability: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
• Concentration: 200μg/mL
• Storage Buffer: 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5% Trehalose and Proclin300.
• Reconstitution: Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0mg/mL. Do not vortex.

Gene Information

• Gene Name: Grpr gastrin releasing peptide receptor [ Mus musculus (house mouse) ]
• Official Symbol: Grpr
• Synonyms: THBS2; thrombospondin 2; TSP2; thrombospondin-2
• Gene ID: 14829
• mRNA Refseq: NM_008177.3
• Protein Refseq: NP_032203.1
• UniProt ID: P21729