Recombinant Mouse Gpa33 Protein, His-tagged, Alexa Fluor 555 conjugated

• Cat.No.: Gpa33-246MAF555
• Product Overview: Alexa Fluor 555 conjugated recombinant Mouse Gpa33 (Leu22-Ile235) protein was fused to His-tag at C-terminus and expressed in human 293 cells (HEK293).

Specification

• Species: Mouse
• Source: HEK293
• Tag: His
• ProteinLength: Leu22-Ile235
• Description: Glycoprotein A33 (GPA33) is also known as Cell surface A33 antigen, is a single-pass type I membrane protein which is expressed in normal gastrointestinal epithelium and in 95% of colon cancers. GPA33 The predicted mature protein has a 213-amino acid extracellular region, a single transmembrane domain, and a 62-amino acid intracellular tail. The sequence of the extracellular region contains 1 Ig-like C2-type (immunoglobulin-like) domain and 1 Ig-like V-type (immunoglobulin-like) domain characteristic of the CD2 subgroup of the immunoglobulin (Ig) superfamily, which contains. GPA33 may play a role in cell-cell recognition and signaling.
• Form: Lyophilized
• Molecular Mass: The protein has a calculated MW of 24.7 kDa. The protein migrates as 33-42 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• N-terminal Sequence Analysis: Leu 22
• Endotoxin: < 1.0 EU/ μg by the LAL method.
• Purity: > 95 % as determined by SDS-PAGE
• Characteristic: Disulfide-linked homodimer
  Labeled with Alexa Fluor 555 via amines
  With an excitation and emission maximum of 555/565 nm, Alexa Fluor 555 can be efficiently excited using a 543 nm He-Ne laser line and detected under standard TRITC/Cy3 filters.
• Storage: For long term storage, the product should be stored at lyophilized state at -20 centigrade or lower.
  Please avoid repeated freeze-thaw cycles.
  This product is stable after storage at:
  -20 to -70 centigrade for 12 months in lyophilized state;
  -70 centigrade for 3 months under sterile conditions after reconstitution.
• Storage Buffer: Lyophilized from 0.22 μm filtered solution in PBS, pH7.4, 10% trehalose.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 μg/μL. Centrifuge the vial at 4 centigrade before opening to recover the entire contents.

Gene Information

• Gene Name: Gpa33
• Official Symbol: Gpa33
• Synonyms: GPA33; glycoprotein A33 (transmembrane); cell surface A33 antigen; mA33; A33 antigen; BB116197; 2010310L10Rik; 2210401D16Rik
• Gene ID: 59290
• mRNA Refseq: NM_021610
• Protein Refseq: NP_067623
• UniProt ID: Q9JKA5