Active Recombinant Cynomolgus GPA33 protein, Fc-tagged

• Cat.No.: GPA33-1482C
• Product Overview: Recombinant Cynomolgus GPA33 protein(Ile22-Val235), fused with Fc tag, was expressed in HEK293.

Specification

• Species: Cynomolgus
• Source: HEK293
• Tag: Fc
• ProteinLength: 22-235 a.a.
• Form: Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, 25 mM Arginine, 150 mM NaCl, pH7.5.
• Bio-activity: Immobilized Cynomolgus GPA33, Fc Tag at 1 μg/mL (100 μL/well) can bind Monoclonal Anti-Human GPA33 Antibody, Human IgG1 with a linear range of 1-5 ng/mL.
• Molecular Mass: This protein carries a human IgG1 Fc tag at the C-terminus.The protein has a calculated MW of 50.2 kDa. The protein migrates as 60-65 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Proteinlength: Ile22-Val235
• Endotoxin: Less than 1.0 EU per μg by the LAL method.
• Purity: >95% as determined by reduced SDS-PAGE. >90% as determined by SEC-MALS.
• Storage: For long term, the product should be stored at lyophilized state at -20°C or lower.Please avoid repeated freeze-thaw cycles. This product is stable after at:
  -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.