Recombinant Mouse Got1, His-tagged

• Cat.No.: Got1-65M
• Product Overview: Recombinant mouse Got1 fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Specification

• Description: Got1 also known as Glutamate oxaloacetate transaminase 1 is a pyridoxal phosphate-dependent enzyme which exists in cytoplasmic and mitochondrial forms, GOT1 and GOT2, respectively. GOT1 plays a role in amino acid metabolism and the urea and tricarboxylic acid cycles. The two enzymes are homodimeric and show close homology.
• Source: E.coli
• Species: Mouse
• Tag: His
• Form: Liquid, In Phosphate buffered saline (pH7.4) containing 10% glycerol, 1mM DTT
• Molecular Mass: 48.6kDa (436aa) confirmed by MALDI-TOF
• AA Sequence: MGSSHHHHHH SSGLVPRGSH MGSMAPPSVF AQVPQAPPVL VFKLTADFRD DPDPRKVNLG VGAYRTDESQ PWVLPVVRKV EQKIANDNSL NHEYLPILGL AEFRSCASRL VLGDNSPAIR ENRVGGVQSL GGTGALRIGA DFLGRWYNGT DNKNTPIYVS SPTWENHNAV FSAAGFKDIR PYCYWDAEKR GLDLQGFLND LENAPEFSIF VLHACAHNPT GTDPTPEQWK QIAAVMQRRF LFPFFDSAYQ GFASGDLEKD AWAIRYFVSE GFELFCAQSF SKNFGLYNER VGNLTVVGKE SDSVLRVLSQ MEKIVRITWS NPPAQGARIV AATLSDPELF KEWKGNVKTM ADRILTMRSE LRARLEALKT PGTWSHITEQ IGMFSFTGLN PKQVEYLVNE KHIYLLPSGR INMCGLTTKN LDYVATSIHE AVTKIQ
• Purity: >95% by SDS - PAGE
• Storage: Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
• Concentration: 0.5mg/ml (determined by Bradford assay)

Gene Information

• Gene Name: Got1 glutamate oxaloacetate transaminase 1, soluble [ Mus musculus ]
• Official Symbol: Got1
• Synonyms: GOT1; glutamate oxaloacetate transaminase 1, soluble; aspartate aminotransferase, cytoplasmic; transaminase A; cytosolic aspartate aminotransferase; Got-1; cAspAT; AI789014
• Gene ID: 14718
• mRNA Refseq: NM_010324
• Protein Refseq: NP_034454
• Pathway: 4-hydroxyproline degradation I, organism-specific biosystem; Alanine and aspartate metabolism, organism-specific biosystem; Alanine, aspartate and glutamate metabolism, organism-specific biosystem; Alanine, aspartate and glutamate metabolism, conserved biosystem; Amino acid synthesis and interconversion (transamination), organism-specific biosystem; Arginine and proline metabolism, organism-specific biosystem; Arginine and proline metabolism, conserved biosystem
• Function: L-aspartate:2-oxoglutarate aminotransferase activity; L-aspartate:2-oxoglutarate aminotransferase activity; L-phenylalanine:2-oxoglutarate aminotransferase activity; carboxylic acid binding; catalytic activity; phosphatidylserine decarboxylase activity; pyridoxal phosphate binding; transaminase activity; transferase activity; transferase activity, transferring nitrogenous groups