Recombinant Mouse Gnas Protein, Myc/DDK-tagged

• Cat.No.: Gnas-3259M
• Product Overview: Purified recombinant protein of mouse full-length GNAS (guanine nucleotide binding protein, alpha stimulating) complex locus (Gnas), with C-terminal MYC/DDK tag, expressed in HEK293T cells.

Specification

• Species: Mouse
• Source: HEK293
• Tag: DDK&Myc
• Description: This locus has a highly complex imprinted expression pattern. It gives rise to maternally, paternally, and biallelically expressed transcripts that are derived from four alternative promoters and 5' exons. Some transcripts contain a differentially methylated region (DMR) at their 5' exons, which is commonly found in imprinted genes and correlates with transcript expression. This gene has an antisense transcript. One of the transcripts produced from this locus, and the antisense transcript, are both paternally expressed noncoding RNAs, and may regulate imprinting in this region. In addition, one of the transcripts contains a second overlapping ORF, which encodes a structurally unrelated protein - Alex. Alternative splicing of downstream exons is also observed, which results in different forms of the stimulatory G-protein alpha subunit, a key element of the classical signal transduction pathway linking receptor-ligand interactions with the activation of adenylyl cyclase and a variety of cellular reponses. Additional transcript variants have been found for this gene, but the full-length nature and/or biological validity of some variants have not been determined.
• Molecular Mass: 46.1 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Concentration: >50 μg/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: Gnas GNAS (guanine nucleotide binding protein, alpha stimulating) complex locus [ Mus musculus (house mouse) ]
• Official Symbol: Gnas
• Synonyms: Gnas; GNAS (guanine nucleotide binding protein, alpha stimulating) complex locus; G; N; P; Ga; Gn; P1; P2; P3; XL; GSP; Gs-; Gsa; Nes; Oed; POH; SCG; GPSA; Nesp; SCG6; Gnas1; Nespl; PHP1A; PHP1B; Gnasxl; Nesp55; Oedsml; Galphas; Oed-Sml; Gs-alpha; 5530400H20Rik; A930027G11Rik; C130027O20Rik; protein ALEX; protein GNAS; protein SCG6 (secretogranin VI); Gs alpha subunit; adenylate cyclase-stimulating G alpha protein; alternative gene product encoded by XL-exon; extra large alphas protein; neuroendocrine secretory protein 55; secretogranin VI; stimulatory G-protein alpha subunit
• Gene ID: 14683
• mRNA Refseq: NM_201616
• Protein Refseq: NP_963910
• UniProt ID: P63094