Recombinant Mouse Glrx2 Protein, Myc/DDK-tagged

• Cat.No.: Glrx2-3230M
• Product Overview: Purified recombinant protein of mouse full-length glutaredoxin 2 (thioltransferase) (Glrx2), with C-terminal MYC/DDK tag, expressed in HEK293T cells.

Specification

• Species: Mouse
• Source: HEK293
• Tag: DDK&Myc
• Description: Glutathione-dependent oxidoreductase that facilitates the maintenance of mitochondrial redox homeostasis upon induction of apoptosis by oxidative stress. Involved in response to hydrogen peroxide and regulation of apoptosis caused by oxidative stress. Acts as a very efficient catalyst of monothiol reactions because of its high affinity for protein glutathione-mixed disulfides. Can receive electrons not only from glutathione (GSH), but also from thioredoxin reductase supporting both monothiol and dithiol reactions. Efficiently catalyzes both glutathionylation and deglutathionylation of mitochondrial complex I, which in turn regulates the superoxide production by the complex. Overexpression decreases the susceptibility to apoptosis and prevents loss of cardiolipin and cytochrome c release.
• Molecular Mass: 14.5 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Concentration: >50 μg/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: Glrx2 glutaredoxin 2 (thioltransferase) [ Mus musculus (house mouse) ]
• Official Symbol: Glrx2
• Synonyms: GLRX2; glutaredoxin 2 (thioltransferase); glutaredoxin-2, mitochondrial; Grx2; AI645710; 1700010P22Rik
• Gene ID: 69367
• mRNA Refseq: NM_023505
• Protein Refseq: NP_075994
• UniProt ID: Q923X4