Recombinant Mouse Fgf21, His-tagged

• Cat.No.: Fgf21-503M
• Product Overview: Total 192 AA. Mw: 21.2 kDa (calculated). N-terminal His-tag, 10 extra AA (highlighted).

Specification

• Species: Mouse
• Source: E.coli
• Tag: His
• Description: Fibroblast growth factor 21 is a protein that in humans is encoded by the FGF21 gene. The protein encoded by this gene is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities and are involved in a variety of biological processes including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion.
• Form: Filtered (0,4 μm) and lyophilized in 0.5 mg/mL in 20mM TRIS, 20mM NaCl, pH 7.5.
• Purity: Purity>95%
• Reconstitution: Add deionized water to prepare a working stock solution of approximately 0.5 mg/mL and let the lyophilized pellet dissolve completely. Product is not sterile! Please filter the product by an appropriate sterile filter before using it in the cell culture. Add DTT (0.2 mM ) and NaCl (0.1-0.15 M) before freezing to prevent potential aggregation.
• Applications: ELISA; Western blotting
• Quality Control Test: BCA to determine quantity of the protein; SDS PAGE to determine purity of the protein; LAL to determine quantity of endotoxin
• Sequences of amino acids: MKHHHHHHAS AYPIPDSSPL LQFGGQVRQR YLYTDDDQDT EAHLEIREDG TVVGAAHRSP ESLLELKALK PGVIQILGVK ASRFLCQQPD GALYGSPHFD PEACSFRELL LEDGYNVYQS EAHGLPLRLP QKDSPNQDAT SWGPVRFLPM PGLLHEPQDQ AGFLPPEPPD VGSSDPLSMV EPLQGRSPSY AS
• Storage: Store lyophilized protein at -20°C. Lyophilized protein remains stable until the expiry date when stored at -20°C. Aliquot reconstituted protein to avoid repeated freezing/thawing cycles and store at -80°C for long term storage. Reconstituted protein can be stored at 4°C for a limited period of time; it does not show any change after one week at 4°C.

Gene Information

• Gene Name: Fgf21 fibroblast growth factor 21 [ Mus musculus ]
• Official Symbol: Fgf21
• Synonyms: Fgf21; fibroblast growth factor 21
• Gene ID: 56636
• mRNA Refseq: NM_020013
• Protein Refseq: NP_064397
• UniProt ID: Q9JJN1
• Chromosome Location: 7B2
• Pathway: ESC Pluripotency Pathways; MAPK signaling pathway; Melanoma; Pathways in cancer; Regulation of Actin Cytoskeleton; Regulation of actin cytoskeleton
• Function: growth factor activity