Recombinant Mouse Fgf15, His-tagged

• Cat.No.: Fgf15-499M
• Product Overview: Total 203 AA. MW: 23.78 kDa (calculated). N-Terminal His-tag, 10 extra AA (highlighted).

Specification

• Species: Mouse
• Source: E.coli
• Tag: His
• Description: Fibroblast growth factor 19 is a protein that in humans is encoded by the FGF19 gene. The protein encoded by this gene is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes including embryonic development cell growth, morphogenesis, tissue repair, tumor growth and invasion. This growth factor is a high affinity, heparin dependent ligand for FGFR4. Expression of this gene was detected only in fetal but not adult brain tissue. Synergistic interaction of the chick homolog and Wnt-8c has been shown to be required for initiation of inner ear development.
• Form: Filtered (0,4 μm) and lyophilized in 0.5 mg/mL in 20mM TRIS, 20mM NaCl, PH 8.0.
• Research topic: Energy metabolism and body weight regulation
• Purity: >90%
• Reconstitution: Add deionized water to prepare a working stock solution of approximately 0.5 mg/mL and let the lyophilized pellet dissolve completely. Product is not sterile! Please filter the product by an appropriate sterile filter before using it in the cell culture.
• Applications: ELISA, Western blotting
• Quality Control Test: BCA to determine quantity of the protein; SDS PAGE to determine purity of the protein; LAL to determine quantity of endotoxin
• Sequences of amino acids: MKHHHHHHAS RPLAQQSQSV SDEDPLFLYG WGKITRLQYL YSAGPYVSNC FLRIRSDGSV DCEEDQNERN LLEFRAVALK TIAIKDVSSV RYLCMSADGK IYGLIRYSEE DCTFREEMDC LGYNQYRSMK HHLHIIFIQA KPREQLQDQK PSNFIPVFHR SFFETGDQLR SKMFSLPLES DSMDPFRMVE DVDHLVKSPS FQK
• Storage: Store lyophilized protein at -20°C. Lyophilized protein remains stable until the expiry date when stored at -20°C. Aliquot reconstituted protein to avoid repeated freezing/thawing cycles and store at -80°C for long term storage. Reconstituted protein can be stored at 4°C for a limited period of time; it does not show any change after one week at 4°C.

Gene Information

• Gene Name: Fgf15 fibroblast growth factor 15 [ Mus musculus ]
• Official Symbol: Fgf15
• Synonyms: Fgf15; fibroblast growth factor 15; FGF19; FGF-15
• Gene ID: 14170
• mRNA Refseq: NM_008003
• Protein Refseq: NP_032029
• Pathway: Downstream signaling of activated FGFR; ESC Pluripotency Pathways; FGFR ligand binding and activation; FGFR1 ligand binding and activation; FGFR1b ligand binding and activation; FGFR1c and Klotho ligand binding and activation; FGFR2b ligand binding and activation; FRS2-mediated cascade; IRS-related events; MAPK signaling pathway; PI-3K cascade; Pathways in cancer; Regulation of actin cytoskeleton; Signaling by FGFR; Signaling by Insulin receptor; Insulin receptor signalling cascade
• Function: fibroblast growth factor receptor binding; growth factor activity