Recombinant Mouse Fbn1 Protein, His-tagged

• Cat.No.: Fbn1-1465M
• Product Overview: Recombinant Mouse Fbn1 Protein (Leu457-Asp634) with N-His tag was expressed in E. coli.

Specification

• Species: Mouse
• Source: E.coli
• Tag: His
• Protein Length: Leu457-Asp634
• Description: This gene encodes a member of the fibrillin family of proteins. The encoded preproprotein is proteolytically processed to generate two proteins including the extracellular matrix component fibrillin-1 and the protein hormone asprosin. Fibrillin-1 is an extracellular matrix glycoprotein that serves as a structural component of calcium-binding microfibrils. Asprosin, secreted by white adipose tissue, has been shown to regulate glucose homeostasis. Homozygous knockout mice for this gene exhibit impaired aortic development and early postnatal death, which was attributed to a deficiency in the fibrillin-1 protein. Mice with a hypomorphic allele of this gene exhibit impaired glucose homeostasis, likely due to a reduction in serum asprosin levels.
• Form: Freeze-dried powder
• Molecular Mass: Predicted Molecular Mass: 13.4 kDa
  Accurate Molecular Mass: 21 kDa
• Purity: > 97%
• Applications: Cell culture; Activity Assays.
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 centigrade for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 centigrade for one month. Aliquot and store at -80 centigrade for 12 months.
• Storage Buffer: 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
• Reconstitution: Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Gene Information

• Gene Name: Fbn1 fibrillin 1 [ Mus musculus (house mouse) ]
• Official Symbol: Fbn1
• Synonyms: FBN1; fibrillin 1; fibrillin-1; tight skin; Tsk; Fib-1; AI536462; B430209H23
• Gene ID: 14118
• mRNA Refseq: NM_007993
• Protein Refseq: NP_032019
• UniProt ID: P35555