Recombinant Mouse F7 protein, His-tagged

• Cat.No.: F7-1912M
• Product Overview: Recombinant Mouse F7 aa. (Ala42~Lys169 (Accession # P70375)) fused with N-terminal His tag was produced in E. coli cells.

Specification

• Description: This gene encodes a vitamin K-dependent serine protease that plays a critical role in the extrinsic pathway of blood coagulation. Upon contact with tissue factor III (TF III), the encoded protein forms an activated complex termed TF-FVIIa that initiates the coagulation cascade involving other coagulation factors, ultimately resulting in a fibrin clot. Complete lack of the encoded protein in mice results in in perinatal lethality due to bleeding from normal blood vessels.
• Source: E. coli
• Species: Mouse
• Tag: His
• Form: Freeze-dried powder
• Molecular Mass: Predicted Molecular Mass: 16.2kDa.
• Protein length: Ala42~Lys169
• Endotoxin: <1.0EU per 1ug (determined by the LAL method)
• Purity: >95%
• Characteristic: The isoelectric point is 5.
• Applications: SDS-PAGE; WB; ELISA; IP.
• Stability: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
• Storage Buffer: Supplied as lyophilized form in 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5% trehalose, and preservative.
• Reconstitution: Reconstitute in ddH2O.

Gene Information

• Gene Name: F7 coagulation factor VII [ Mus musculus (house mouse) ]
• Official Symbol: F7
• Synonyms: Pfkl; phosphofructokinase, liver type; PFK-L; ATP-PFK; ATP-dependent 6-phosphofructokinase, liver type; 6-phosphofructokinase type B; 6-phosphofructokinase, liver type; PFK-B; phosphofructo-1-kinase isozyme B; phosphofructokinase 1; phosphofructokinase, liver, B-type; phosphohexokinase
• Gene ID: 14068
• mRNA Refseq: NM_010172.4
• Protein Refseq: NP_034302.2
• UniProt ID: P70375