Recombinant Mouse F5 protein, His-tagged

• Cat.No.: F5-770M
• Product Overview: Recombinant Mouse F5 aa. (Pro364~Cys611 (Accession # O88783)) fused with N-terminal His tag was produced in E. coli cells.

Specification

• Description: This gene encodes a glycoprotein coagulation factor that plays a critical role in the process of blood coagulation and hemostasis. The encoded protein is activated by thrombin, to generate a heterodimer containing heavy and light chains held together by calcium ions. About half of the mice lacking the encoded protein die at an embryonic stage possible due to abnormal yolk-sac vasculature while the remaining animals succumbed to massive hemorrhage immediately after birth. A point mutation in this gene has been shown to cause disseminated intravascular thrombosis in the perinatal period, resulting in frequent deaths of newborn mice.
• Source: E. coli
• Species: Mouse
• Tag: His
• Form: Freeze-dried powder
• Molecular Mass: Predicted Molecular Mass: 30.1kDa
• Protein length: Pro364~Cys611
• Endotoxin: <1.0EU per 1µg (determined by the LAL method)
• Purity: >95%
• Characteristic: The isoelectric point is 9.5.
• Applications: SDS-PAGE; WB; ELISA; IP.
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
• Storage buffer: Supplied as lyophilized form in PBS, pH7.4, containing 5% sucrose, 0.01% sarcosyl.
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.

Gene Information

• Gene Name: F5 coagulation factor V [ Mus musculus (house mouse) ]
• Official Symbol: F5
• Synonyms: Cf5; Cf-5; AI173222; activated protein C cofactor; coagulation factor V
• Gene ID: 14067
• mRNA Refseq: NM_007976.3
• Protein Refseq: NP_032002.1
• UniProt ID: O88783