Recombinant Mouse Erbb3 protein(Met1-His641), His-tagged

• Cat.No.: Erbb3-2515M
• Product Overview: Recombinant Mouse ERBB3 (Q61526) (Met 1-His 641) was expressed in HEK293, with a C-terminal polyhistidine tag.

Specification

• Source: HEK293
• Species: Mouse
• Tag: His
• Protein length: 1-641 a.a.
• Form: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Bio-activity: Measured by its binding ability in a functional ELISA.1. Immobilized mouse ErbB3-His at 10 μg/ml (100 μl/well) can bind biotinylated human NRG1 (isoform Beta1) with a linear range of 0.018-0.125 μg/ml. 2. Immobilized mouse ErbB3-His at 10 μg/ml (100 μl/well) can bind biotinylated human NRG1 (aa 2-246)-Fc with a linear range of 6.25-100 ng/ml.
• Molecular Mass: The secreted recombinant mouse ERBB3 comprises 633 amino acids and has a calculated molecular mass of 70 kDa. As a result of glycosylation, the apparent molecular mass of the recombinant protein is approximately 95 kDa in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 93 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: Erbb3 v-erb-b2 erythroblastic leukemia viral oncogene homolog 3 (avian) [ Mus musculus ]
• Official Symbol: Erbb3
• Synonyms: ERBB3; v-erb-b2 erythroblastic leukemia viral oncogene homolog 3 (avian); receptor tyrosine-protein kinase erbB-3; c-erbB-3; receptor tyrosine kinase; glial growth factor receptor; proto-oncogene-like protein c-ErbB-3; avian erythroblastosis oncogene B 3 receptor; Her3; C76256; Erbb-3; Erbb3r; MGC117742
• Gene ID: 13867
• mRNA Refseq: NM_010153
• Protein Refseq: NP_034283