Recombinant Human ERBB3 Protein, His/GST-tagged, Alexa Fluor 555 conjugated

• Cat.No.: ERBB3-7276HAF555
• Product Overview: Alexa Fluor 555 conjugated recombinant human ERBB3 (NP_001973.2) cytoplasmic domain (Pro 730-Ser 1065), fused with the N-terminal polyhistidine-tagged GST tag, was produced in Baculovirus-Insect cells.

Specification

• Source: Insect cells
• Species: Human
• Tag: His&GST
• Form: Lyophilized
• Molecular Mass: The recombinant human ErbB3 /GST chimera consists of 573 amino acids and has a calculated molecular mass of 65 kDa. It migrates as an approximately 65 kDa band as predicted in SDS-PAGE under reducing conditions.
• Protein length: 573
• Endotoxin: < 1.0 EU/ μg of the protein as determined by the LAL method.
• Characteristic: Disulfide-linked homodimer
  Labeled with Alexa Fluor 555 via amines
  With an excitation and emission maximum of 555/565 nm, Alexa Fluor 555 can be efficiently excited using a 543 nm He-Ne laser line and detected under standard TRITC/Cy3 filters.
• Stability: Samples are stable for up to 12 months from date of receipt at -70 centigrade.
• Storage: Store it under sterile conditions at -20 to -70 centigrade. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Storage Buffer: Lyophilized from 0.2 μm filtered solution of 50 mM Tris, 100 mM NaCl, pH 7.5, 10% gly, 1 mM GSH
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution. Centrifuge the vial at 4 centigrade before opening to recover the entire contents.

Gene Information

• Gene Name: ERBB3 v-erb-b2 erythroblastic leukemia viral oncogene homolog 3 (avian) [ Homo sapiens ]
• Official Symbol: ERBB3
• Gene ID: 2065
• mRNA Refseq: NM_001005915
• Protein Refseq: NP_001005915
• MIM: 190151
• UniProt ID: P21860