Recombinant Mouse ERBB2 Protein, His-tagged

• Cat.No.: ERBB2-288M
• Product Overview: Recombinant Mouse ERBB2 protein with His tag was expressed in HEK293.

Specification

• Species: Mouse
• Source: HEK293
• Tag: His
• Protein Length: 711
• Description: Predicted to enable several functions, including ErbB-3 class receptor binding activity; Hsp90 protein binding activity; and enzyme binding activity. Predicted to contribute to growth factor binding activity. Involved in positive regulation of GTPase activity and positive regulation of protein phosphorylation. Acts upstream of or within several processes, including negative regulation of immature T cell proliferation in thymus; nervous system development; and neuromuscular junction development. Located in apical plasma membrane; cytoplasmic vesicle; and myelin sheath. Is expressed in several structures, including adrenal gland; alimentary system; central nervous system; genitourinary system; and sensory organ. Used to study Hirschsprung's disease; breast cancer; and dilated cardiomyopathy. Human ortholog(s) of this gene implicated in carcinoma (multiple); high grade glioma; reproductive organ cancer (multiple); and urinary bladder cancer. Orthologous to human ERBB2 (erb-b2 receptor tyrosine kinase 2).
• Form: Lyophilized
• Molecular Mass: 70.5 kDa
• AA Sequence: MELAAWCRWGFLLALLSPGAAGTQVCTGTDMKLRLPASPETHLDMLRHLYQGCQVVQGNLELTYLPANASLSFLQDIQEVQGYMLIAHNRVKHVPLQRLRIVRGTQLFEDKYALAVLDNRDPLDNVTTAAPGRTPEGLRELQLRSLTEILKGGVLIRGNPQLCYQDMVLWKDVLRKNNQLAPVDMDTNRSRACPPCAPTCKDNHCWGESPEDCQILTGTICTSGCARCKGRLPTDCCHEQCAAGCTGPKHSDCLACLHFNHSGICELHCPALITYNTDTFESMLNPEGRYTFGASCVTTCPYNYLSTEVGSCTLVCPPNNQEVTAEDGTQRCEKCSKPCAGVCYGLGMEHLRGARAITSDNIQEFAGCKKIFGSLAFLPESFDGNPSSGVAPLKPEHLQVFETLEEITGYLYISAWPESFQDLSVFQNLRVIRGRILHDGAYSLTLQGLGIHSLGLRSLRELGSGLALIHRNTHLCFVNTVPWDQLFRNPHQALLHSGNRPEEACGLEGLVCNSLCARGHCWGPGPTQCVNCSQFLRGQECVEECRVWKGLPREYVRGKHCLPCHPECQPQNSSETCYGSEADQCEACAHYKDSSSCVARCPSGVKPDLSYMPIWKYPDEEGICQPCPINCTHSCVDLDERGCPAEQRASPVTFIIATVVGVLLFLIIVVVIGILIKRRRQKIRKYTMRRLLQETEVRRAEGLLAPPWLCS
• Purity: > 98%
• Applications: WB; ELISA; FACS; FC
• Stability: This bioreagent is stable at 4 centigrade (short-term) and -70 centigrade(long-term). After reconstitution, sample may be stored at 4 centigrade for 2-7 days and below -18 centigrade for future use.
• Storage: At -20 centigrade.
• Concentration: 1 mg/mL
• Storage Buffer: PBS (pH 7.4-7.5). Sterile-filtered colorless solution.
• Reconstitution: Reconstitute in sterile distilled H2O to no less than 100 μg/mL; dilute reconstituted stock further in other aqueous solutions if needed. Please review COA for lot-specific instructions. Final measurements should be determined by the end-user for optimal performance.

Gene Information

• Gene Name: Erbb2 v-erb-b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog (avian) [ Mus musculus (house mouse) ]
• Official Symbol: ERBB2
• Synonyms: ERBB2; v-erb-b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog (avian); receptor tyrosine-protein kinase erbB-2; c-erbB-2; p185erbB2; Neu oncogene; proto-oncogene NEU; proto-oncogene c-ErbB-2; avian erythroblastosis oncogene B 2; Neu; HER2; HER-2; c-neu; Erbb-2; c-erbB2; mKIAA3023
• Gene ID: 13866
• mRNA Refseq: NM_001003817
• Protein Refseq: NP_001003817
• UniProt ID: P70424

Q&As

Do you have a recommended protocol for immobilizing any avi-tagged recombinant proteins on a streptavidin coated plate?

1.Prepare the streptavidin-coated plate by washing it with PBS (phosphate-buffered saline) or another suitable buffer to remove any impurities. 2.Dilute the Avi-tagged protein in the desired buffer at the desired concentration. It is important to use a buffer that is compatible with the protein and does not interfere with its activity or stability. 3.Add the diluted protein to the streptavidin-coated plate and incubate it for a suitable amount of time at room temperature or 4°C. The exact conditions will depend on the specific protein and the desired level of immobilization. 4.Wash the plate with a suitable buffer to remove any unbound protein. 5.Block the remaining binding sites on the plate with a suitable blocking agent such as BSA (bovine serum albumin) or casein. 6.Wash the plate again to remove any unbound blocking agent. 7.Use the immobilized protein for further experiments such as ELISA, Western blotting, or other assays.