Recombinant Mouse Erap1 Protein, Myc/DDK-tagged

• Cat.No.: Erap1-2851M
• Product Overview: Purified recombinant protein of mouse full-length endoplasmic reticulum aminopeptidase 1 (Erap1), with C-terminal MYC/DDK tag, expressed in HEK293T cells.

Specification

• Description: Aminopeptidase that plays a central role in peptide trimming, a step required for the generation of most HLA class I-binding peptides. Peptide trimming is essential to customize longer precursor peptides to fit them to the correct length required for presentation on MHC class I molecules. Strongly prefers substrates 9-16 residues long. Rapidly degrades 13-mer to a 9-mer and then stops. Preferentially hydrolyzes the residue Leu and peptides with a hydrophobic C-terminus, while it has weak activity toward peptides with charged C-terminus. May play a role in the inactivation of peptide hormones. May be involved in the regulation of blood pressure through the inactivation of angiotensin II and/or the generation of bradykinin in the kidney.
• Source: HEK293T
• Species: Mouse
• Tag: Myc&DDK
• Molecular Mass: 107 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Concentration: >50 μg/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: Erap1 endoplasmic reticulum aminopeptidase 1 [ Mus musculus (house mouse) ]
• Official Symbol: Erap1
• Synonyms: ERAP1; endoplasmic reticulum aminopeptidase 1; A-LAP; ARTS-1; PILS-AP; aminopeptidase PILS; VEGF-induced aminopeptidase; adipocyte-derived leucine aminopeptidase; puromycin-insensitive leucyl-specific aminopeptidase; type 1 tumor necrosis factor receptor shedding aminopeptidase regulator; Arts1; ERAAP; PILSA; PILSAP
• Gene ID: 80898
• mRNA Refseq: NM_030711
• Protein Refseq: NP_109636
• UniProt ID: Q9EQH2