Recombinant Mouse Eogt Protein, His-tagged

• Cat.No.: Eogt-48M
• Product Overview: Recombinant mouse EOGT, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Specification

• Species: Mouse
• Source: Insect Cells
• Tag: His
• Description: EOGT, also known as EGF domain-specific O-linked N-acetylglucosamine transferase, is involved in the regulation of Notch receptor. O-GlcNAc (O-linked b-N-acetylglucosamine) is introduced by a single intracellular O-GlcNAc transferase (OGT) and a single extracellular O-GlcNAc transferase (EOGT). O-GlcNAc results from the addition of a single N-acetylglucosamine residue to serine/threonine residues.
• Form: Liquid. In Phosphate Buffered Saline (pH 7.4) containing 10% glycerol.
• Molecular Mass: 60.4kDa (516aa)50-70KDa (SDS–PAGE under reducing conditions.)
• AA Sequence: DKAHSEADDA PGKALYDYSS LRLPAEHIPF FLHNNRHVAS VCREDSHCPY KKHLENLNYC WGYEKSCAPE FRFGSPVCSY VDLGWTDTLESAQDMFWRQA DFGYARERLG EIRTICQPER ASDSSLVCSR YLQYCRATGL YLDLRNIKRN HDRFKEDFLQ GGEIGGYCKL DSHALVSEGQRKSPLQSWFA ELQGYTQLNF RPIEDAKCDI VVEKPTYFMK LDAGINMYHH FCDFLNLYLT QHVNNSFSTD VYIVMWDTST YGYGDLFSDTWKAFTDYDVI HLKTYDSKKV CFKEAVFSLL PRMRYGLFYN TPLISGCQNT GLFRAFSQHV LHRLNITQEG PKDGKVRVTI LARSTEYRKILNQDELVNAL KTVSTFEVRV VDYKYRELGF LDQLRITHNT DIFIGMHGAG LTHLLFLPDW AAVFELYNCE DERCYLDLAR LRGIHYITWRKPSKVFPQDK GHHPTLGEHP KFTNYSFDVE EFMYLVLQAA EHVLQHPQWP FKKKHDELLE HHHHHH
• Endotoxin: < 1.0 EU per 1 microgram of protein (determined by LAL method)
• Purity: >85% by SDS - PAGE
• Storage: Can be stored at +4centigrade short term (1-2 weeks). For long term storage, aliquot and store at -20Centigrade or -70Centigrade. Avoid repeated freezing and thawing cycles.
• Concentration: 0.5mg/ml (determined by Absorbance at 280nm)

Gene Information

• Gene Name: Eogt EGF domain-specific O-linked N-acetylglucosamine (GlcNAc) transferase [ Mus musculus ]
• Official Symbol: Eogt
• Synonyms: EOGT; A130022J15RiK; Aer61; AI447490; AW214473
• Gene ID: 101351
• mRNA Refseq: NM_175313
• Protein Refseq: NP_780522
• UniProt ID: Q8BYW9
• Chromosome Location: 6; 6 D3
• Pathway: Other types of O-glycan biosynthesis, organism-specific biosystem; Other types of O-glycan biosynthesis, conserved biosystem
• Function: protein N-acetylglucosaminyltransferase activity; transferase activitytransferase activity, transferring glycosyl groups