Recombinant Mouse Ensa Protein, Myc/DDK-tagged

• Cat.No.: Ensa-2834M
• Product Overview: Purified recombinant protein of mouse full-length endosulfine alpha (Ensa), with C-terminal MYC/DDK tag, expressed in HEK293T cells.

Specification

• Species: Mouse
• Source: HEK293
• Tag: DDK&Myc
• Description: Protein phosphatase inhibitor that specifically inhibits protein phosphatase 2A (PP2A) during mitosis. When phosphorylated at Ser-67 during mitosis, specifically interacts with PPP2R2D (PR55-delta) and inhibits its activity, leading to inactivation of PP2A, an essential condition to keep cyclin-B1-CDK1 activity high during M phase. Also acts as a stimulator of insulin secretion by interacting with sulfonylurea receptor (ABCC8), thereby preventing sulfonylurea from binding to its receptor and reducing K(ATP) channel currents.
• Molecular Mass: 12.9 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Concentration: >50 μg/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: Ensa endosulfine alpha [ Mus musculus (house mouse) ]
• Official Symbol: Ensa
• Synonyms: ENSA; endosulfine alpha; alpha-endosulfine; ARPP-19e; AI451924; 1700020C18Rik; 2610007F17Rik
• Gene ID: 56205
• mRNA Refseq: NM_001026212
• Protein Refseq: NP_001021383
• UniProt ID: P60840