Recombinant Mouse Enpp1 Protein, Myc/DDK-tagged

• Cat.No.: Enpp1-2831M
• Product Overview: Purified recombinant protein of mouse full-length ectonucleotide pyrophosphatase/phosphodiesterase 1 (Enpp1), with C-terminal MYC/DDK tag, expressed in HEK293T cells.

Specification

• Description: This gene encodes a member of the nucleoside pyrophosphatase/phosphodiesterase family of enzymes that catalyzes the hydrolysis of pyrophosphate and phosphodiester bonds in nucleotide triphosphates and oligonucleotides, respectively, to generate nucleoside 5'-monophosphates. The encoded protein is a type II transmembrane glycoprotein that negatively regulates bone mineralization. Mice harboring a nonsense mutation in this gene, termed tiptoe walking (ttw), exhibit ectopic ossification of the spinal ligaments. The encoded protein binds to the insulin receptor, inhibits downstream signaling events and induces insulin resistance and glucose tolerance. This gene is located adjacent to a paralog on chromosome 10. Alternative splicing results in multiple transcript variants encoding different isoforms.
• Source: HEK293T
• Species: Mouse
• Tag: Myc&DDK
• Molecular Mass: 103.5 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Concentration: >50 μg/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: Enpp1 ectonucleotide pyrophosphatase/phosphodiesterase 1 [ Mus musculus (house mouse) ]
• Official Symbol: Enpp1
• Synonyms: ENPP1; ectonucleotide pyrophosphatase/phosphodiesterase 1; ectonucleotide pyrophosphatase/phosphodiesterase family member 1; E-NPP 1; tiptoe walking; lymphocyte antigen 41; plasma-cell membrane glycoprotein PC-1; phosphodiesterase I/nucleotide pyrophosphatase 1; 1 ectonucleotide pyrophosphatase/phosphodiesterase 1; Pca; ttw; twy; M6S1; NPP1; Npps; PC-1; Ly-41; Pca-1; Pdnp1; C76301; CD203c; E-NPP1; AI428932; 4833416E15Rik
• Gene ID: 18605
• mRNA Refseq: NM_008813
• Protein Refseq: NP_032839
• UniProt ID: G3X9S2