Recombinant Mouse Egfr protein, His-tagged

• Cat.No.: Egfr-5224M
• Product Overview: Recombinant Mouse Egfr (Leu25-Ser647) protein was fused to His-tag at C-terminus and expressed in human 293 cells (HEK293).

Specification

• Species: Mouse
• Source: HEK293
• Tag: His
• Protein Length: Leu25-Ser647
• Description: The epidermal growth factor receptor (EGFR; ErbB-1; HER1) is the cell-surface receptor for members of the epidermal growth factor family (EGF-family) of extracellular protein ligands. The epidermal growth factor receptor is a member of the ErbB family of receptors, a subfamily of four closely related receptor tyrosine kinases: EGFR (ErbB-1), HER2/c-neu (ErbB-2), Her 3 (ErbB-3) and Her 4 (ErbB-4). Mutations affecting EGFR expression or activity could result in cancer.
• Predicted N Terminal: Leu25
• Form: Lyophilized from 0.22 μm filtered solution in PBS, pH7.4, 10% trehalose.
• Molecular Mass: The protein has a calculated MW of 71.2 kDa. The protein migrates as 86-110 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Endotoxin: Less than 1.0 EU per μg by the LAL method.
• Purity: >95% as determined by SDS-PAGE.
• Storage: For long term storage, the product should be stored at lyophilized state at -20 centigrade or lower.
  Please avoid repeated freeze-thaw cycles.
  This product is stable after storage at:
  -20 to -70 centigrade for 12 months in lyophilized state;
  -70 centigrade for 3 months under sterile conditions after reconstitution.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4℃ before opening to recover the entire contents.

Gene Information

• Gene Name: Egfr
• Official Symbol: Egfr
• Synonyms: EGFR; epidermal growth factor receptor; waved 2; avian erythroblastic leukemia viral (v-erb-b) oncogene homolog; Wa5; wa2; Erbb; Errp; wa-2; AI552599; 9030024J15Rik
• Gene ID: 13649
• mRNA Refseq: NM_007912
• Protein Refseq: NP_031938
• UniProt ID: Q01279