Recombinant Mouse Edn3 Protein, His&GST-tagged

• Cat.No.: Edn3-1404M
• Product Overview: Recombinant Mouse Edn3 Protein (Ala17-Pro214) with N-His&GST tag was expressed in E. coli.

Specification

• Species: Mouse
• Source: E.coli
• Tag: GST&His
• Protein Length: Ala17-Pro214
• Description: This gene is a member of the endothelin family whose members encode proteins that act on G protein-coupled receptors. Endothelins are produced as large prepropolypeptide precursors that undergo a first cleavage by a subtilisin serine protease to form an inactive intermediate, which in turn is cleaved again by endothelin-converting enzyme 1 (ECE-1) to yield the active 21 amino acid peptide. This gene encodes a protein which is expressed in neural crest cells (NCC), binds to endothelin receptor b (Ednrb) and plays an essential role in the development of NCC-derived cell lineages including melanocytes and enteric neurons. Mutations in this gene are associated with terminal aganglionosis and white spotted coat in mice and Hirschsprung's disease and Waardenburg syndrome in humans.
• Form: Freeze-dried powder
• Molecular Mass: Predicted Molecular Mass: 51.6 kDa
  Accurate Molecular Mass: 52 kDa
• Purity: > 90%
• Applications: Positive Control; Immunogen; SDS-PAGE; WB.
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 centigrade for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 centigrade for one month. Aliquot and store at -80 centigrade for 12 months.
• Storage Buffer: PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
• Reconstitution: Reconstitute in PBS or others.

Gene Information

• Gene Name: Edn3 endothelin 3 [ Mus musculus (house mouse) ]
• Official Symbol: Edn3
• Synonyms: EDN3; endothelin 3; endothelin-3; ET-3; PPET3; lethal spotting; preproendothelin-3; ls; tmgc48; 114CH19; 114-CH19
• Gene ID: 13616
• mRNA Refseq: NM_007903
• Protein Refseq: NP_031929
• UniProt ID: P48299