Recombinant Mouse Dtd1 Protein, Myc/DDK-tagged

• Cat.No.: Dtd1-2667M
• Product Overview: Purified recombinant protein of mouse full-length D-tyrosyl-tRNA deacylase 1 (Dtd1), with C-terminal MYC/DDK tag, expressed in HEK293T cells.

Specification

• Species: Mouse
• Source: HEK293
• Tag: DDK&Myc
• Description: An aminoacyl-tRNA editing enzyme that deacylates mischarged D-aminoacyl-tRNAs. Also deacylates mischarged glycyl-tRNA(Ala), protecting cells against glycine mischarging by AlaRS. Acts via tRNA-based rather than protein-based catalysis; rejects L-amino acids rather than detecting D-amino acids in the active site. By recycling D-aminoacyl-tRNA to D-amino acids and free tRNA molecules, this enzyme counteracts the toxicity associated with the formation of D-aminoacyl-tRNA entities in vivo and helps enforce protein L-homochirality.
• Molecular Mass: 23.4 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Concentration: >50 μg/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: Dtd1 D-tyrosyl-tRNA deacylase 1 [ Mus musculus (house mouse) ]
• Official Symbol: Dtd1
• Synonyms: Dtd1; D-tyrosyl-tRNA deacylase 1; Ha; DTD; DUE-B; Hars2; 0610006H08Rik; D-aminoacyl-tRNA deacylase 1; D-tyrosyl-tRNA deacylase 1 homolog; D-tyrosyl-tRNA(Tyr) deacylase 1; DNA-unwinding element-binding protein B; gly-tRNA(Ala) deacylase; EC 3.1.1.96
• Gene ID: 66044
• mRNA Refseq: NM_025314
• Protein Refseq: NP_079590
• UniProt ID: Q9DD18