Recombinant Mouse Dnmt3b Protein, Myc/DDK-tagged

• Cat.No.: Dnmt3b-2622M
• Product Overview: Purified recombinant protein of mouse full-length DNA methyltransferase 3B (Dnmt3b), with C-terminal MYC/DDK tag, expressed in HEK293T cells.

Specification

• Species: Mouse
• Source: HEK293
• Tag: DDK&Myc
• Description: This is one of two related genes encoding de novo DNA methyltransferases, which are responsible for the establishment of DNA methylation patterns in embryos. Loss of function of this gene results in severe developmental defects and loss of viability. Mutation of the related gene in humans causes immunodeficiency-centromeric instability-facial anomalies (ICF) syndrome. There is a pseudogene for this gene located adjacent to this gene in the same region of chromosome 2. Alternatively spliced transcript variants encoding multiple isoforms have been observed.
• Molecular Mass: 97.7 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Concentration: >50 μg/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: Dnmt3b DNA methyltransferase 3B [ Mus musculus (house mouse) ]
• Official Symbol: Dnmt3b
• Synonyms: DNMT3B; DNA methyltransferase 3B; DNA (cytosine-5)-methyltransferase 3B; m.MmuIIIB; DNA MTase MmuIIIB; DNA methyltransferase MmuIIIB; MGC124407
• Gene ID: 13436
• mRNA Refseq: NM_001003961
• Protein Refseq: NP_001003961
• UniProt ID: O88509