Recombinant Mouse Dnaja1 Protein, MYC/DDK-tagged

• Cat.No.: Dnaja1-1185M
• Product Overview: Purified recombinant protein of full-length mouse DnaJ heat shock protein family (Hsp40) member A1 (Dnaja1), with C-terminal MYC/DDK tag, was expressed in HEK293T cells.

Specification

• Description: The protein encoded by this gene is a member of the DnaJ family, whose members act as cochaperones of heat shock protein 70. Heat shock proteins facilitate protein folding, trafficking, prevention of aggregation, and proteolytic degradation. Members of this family are characterized by a highly conserved N-terminal J domain, a glycine/phenylalanine-rich region, four CxxCxGxG zinc finger repeats, and a C-terminal substrate-binding domain. The J domain mediates the interaction with heat shock protein 70 to recruit substrates and regulate ATP hydrolysis activity. Mice deficient for this gene display reduced levels of activation‐induced deaminase, an enzyme that deaminates deoxycytidine at the immunoglobulin genes during immune responses. In addition, mice lacking this gene exhibit severe defects in spermatogenesis. Several pseudogenes of this gene are found on other chromosomes. Alternative splicing results in multiple transcript variants.
• Source: HEK293T
• Species: Mouse
• Tag: Myc&DDK
• Molecular Mass: 44.9 kDa
• Purity: >80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Concentration: >50 µg/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: Dnaja1 DnaJ heat shock protein family (Hsp40) member A1 [ Mus musculus (house mouse) ]
• Official Symbol: Dnaja1
• Synonyms: Dnaja1; DnaJ heat shock protein family (Hsp40) member A1; Hsj2; HSJ-2; Nedd7; dnaJ homolog subfamily A member 1; DnaJ (Hsp40) homolog, subfamily A, member 1; dnaJ protein homolog 2; heat shock 40 kDa protein 4; heat shock protein J2; heat shock protein, Dnaj-like 2; neural precursor cell expressed, developmentally down-regulated gene 7
• Gene ID: 15502
• mRNA Refseq: NM_001164671
• Protein Refseq: NP_001158143
• UniProt ID: P63037