Recombinant Mouse Dgka Protein, Myc/DDK-tagged

• Cat.No.: Dgka-2534M
• Product Overview: Purified recombinant protein of mouse full-length diacylglycerol kinase, alpha (Dgka), with C-terminal MYC/DDK tag, expressed in HEK293T cells.

Specification

• Species: Mouse
• Source: HEK293
• Tag: DDK&Myc
• Description: Diacylglycerol kinase that converts diacylglycerol/DAG into phosphatidic acid/phosphatidate/PA and regulates the respective levels of these two bioactive lipids. Thereby, acts as a central switch between the signaling pathways activated by these second messengers with different cellular targets and opposite effects in numerous biological processes. Also plays an important role in the biosynthesis of complex lipids. Can also phosphorylate 1-alkyl-2-acylglycerol in vitro as efficiently as diacylglycerol provided it contains an arachidonoyl group. Also involved in the production of alkyl-lysophosphatidic acid, another bioactive lipid, through the phosphorylation of 1-alkyl-2-acetyl glycerol.
• Molecular Mass: 82.8 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Concentration: >50 μg/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: Dgka diacylglycerol kinase, alpha [ Mus musculus (house mouse) ]
• Official Symbol: Dgka
• Synonyms: DGKA; diacylglycerol kinase, alpha; diacylglycerol kinase alpha; DGK-alpha; DAG kinase alpha; diglyceride kinase alpha; 80 kDa diacylglycerol kinase; 80kDa; Dagk1; AW146112
• Gene ID: 13139
• mRNA Refseq: NM_016811
• Protein Refseq: NP_058091
• UniProt ID: O88673