Recombinant Mouse Dgcr8 Protein, Myc/DDK-tagged

• Cat.No.: Dgcr8-2533M
• Product Overview: Purified recombinant protein of mouse full-length DGCR8, microprocessor complex subunit (Dgcr8), with C-terminal MYC/DDK tag, expressed in HEK293T cells.

Specification

• Species: Mouse
• Source: HEK293
• Tag: DDK&Myc
• Description: Component of the microprocessor complex that acts as a RNA- and heme-binding protein that is involved in the initial step of microRNA (miRNA) biogenesis. Component of the microprocessor complex that is required to process primary miRNA transcripts (pri-miRNAs) to release precursor miRNA (pre-miRNA) in the nucleus. Within the microprocessor complex, DGCR8 function as a molecular anchor necessary for the recognition of pri-miRNA at dsRNA-ssRNA junction and directs DROSHA to cleave 11 bp away form the junction to release hairpin-shaped pre-miRNAs that are subsequently cut by the cytoplasmic DICER to generate mature miRNAs. The heme-bound DGCR8 dimer binds pri-miRNAs as a cooperative trimer (of dimers) and is active in triggering pri-miRNA cleavage, whereas the heme-free DGCR8 monomer binds pri-miRNAs as a dimer and is much less active. Both double-stranded and single-stranded regions of a pri-miRNA are required for its binding. Specifically recognizes and binds N6-methyladenosine (m6A)-containing pri-miRNAs, a modification required for pri-miRNAs processing. Involved in the silencing of embryonic stem cell self-renewal.
• Molecular Mass: 86.8 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Concentration: >50 μg/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: Dgcr8 DGCR8, microprocessor complex subunit [ Mus musculus (house mouse) ]
• Official Symbol: Dgcr8
• Synonyms: DGCR8; DiGeorge syndrome critical region gene 8; microprocessor complex subunit DGCR8; diGeorge syndrome critical region 8 homolog; Gy1; N41; D16Wis2; Vo59c07; D16H22S788E; D16H22S1742E
• Gene ID: 94223
• mRNA Refseq: NM_033324
• Protein Refseq: NP_201581
• UniProt ID: Q9EQM6