Recombinant Mouse Dbt Protein, Myc/DDK-tagged

• Cat.No.: Dbt-2467M
• Product Overview: Purified recombinant protein of mouse full-length dihydrolipoamide branched chain transacylase E2 (Dbt), with C-terminal MYC/DDK tag, expressed in HEK293T cells.

Specification

• Species: Mouse
• Source: HEK293
• Tag: DDK&Myc
• Description: The branched-chain alpha-keto dehydrogenase complex catalyzes the overall conversion of alpha-keto acids to acyl-CoA and CO(2). It contains multiple copies of three enzymatic components: branched-chain alpha-keto acid decarboxylase (E1), lipoamide acyltransferase (E2) and lipoamide dehydrogenase (E3). Within this complex, the catalytic function of this enzyme is to accept, and to transfer to coenzyme A, acyl groups that are generated by the branched-chain alpha-keto acid decarboxylase component.
• Molecular Mass: 53.3 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Concentration: >50 μg/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: Dbt dihydrolipoamide branched chain transacylase E2 [ Mus musculus (house mouse) ]
• Official Symbol: Dbt
• Synonyms: DBT; dihydrolipoamide branched chain transacylase E2; lipoamide acyltransferase component of branched-chain alpha-keto acid dehydrogenase complex, mitochondrial; BCKADE2; BCKAD E2; BCKAD-E2; part of the BCKAD complex; dihydrolipoyl transacylase; branched-chain alpha-ketoacid dehydrogenase, E2 subunit; dihydrolipoyllysine-residue (2-methylpropanoyl)transferase; branched-chain alpha-keto acid dehydrogenase complex component E2; dihydrolipoamide acetyltransferase component of branched-chain alpha-keto acid dehydrogenase complex; D3Wsu60e
• Gene ID: 13171
• mRNA Refseq: NM_010022
• Protein Refseq: NP_034152
• UniProt ID: P53395