Recombinant Mouse Dag1 Protein, His-tagged

• Cat.No.: Dag1-689M
• Product Overview: Recombinant Mouse Dag1(His28~Pro406) fused with His tag at N-terminal was expressed in E. coli.

Specification

• Species: Mouse
• Source: E.coli
• Tag: His
• Protein Length: His28~Pro406
• Description: This gene encodes dystroglycan, a central component of dystrophin-glycoprotein complex that links the extracellular matrix and the cytoskeleton in the skeletal muscle. The encoded preproprotein undergoes O- and N-glycosylation, and proteolytic processing to generate alpha and beta subunits. A complete lack of the encoded protein in mice results in embryonic lethality due to the disorganization of Reichert's membrane. Chimeric mice deficient in the encoded protein overcome embryonic lethality but develop a progressive muscular dystrophy. Alternative splicing results in multiple transcript variants, all encoding the same protein.
• Form: PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
• Molecular Mass: 44.1kDa
• Endotoxin: <1.0EU per 1ug (determined by the LAL method)
• Purity: > 90%
• Applications: Positive Control; Immunogen; SDS-PAGE; WB.
  If bio-activity of the protein is needed, please check active protein
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 centigrade for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 centigrade for one month. Aliquot and store at -80 centigrade for 12 months.
• Reconstitution: Reconstitute in PBS or others

Gene Information

• Gene Name: Dag1 dystroglycan 1 [ Mus musculus ]
• Official Symbol: Dag1
• Synonyms: DAG1; dystroglycan 1; dystroglycan; beta-dystroglycan; alpha-dystroglycan; dystrophin associated glycoprotein 1; dystrophin-associated glycoprotein 1; DG; Dp71; Dp427; D9Wsu13e
• Gene ID: 13138
• mRNA Refseq: NM_010017
• Protein Refseq: NP_034147