Recombinant Mouse Cyp4x1 Protein, Myc/DDK-tagged

• Cat.No.: Cyp4x1-2423M
• Product Overview: Purified recombinant protein of mouse full-length cytochrome P450, family 4, subfamily x, polypeptide 1 (Cyp4x1), with C-terminal MYC/DDK tag, expressed in HEK293T cells.

Specification

• Species: Mouse
• Source: HEK293
• Tag: DDK&Myc
• Description: A cytochrome P450 monooxygenase that selectively catalyzes the epoxidation of the last double bond of the arachidonoyl moiety of anandamide, potentially modulating endocannabinoid signaling. Has no hydroxylase activity toward various fatty acids, steroids and prostaglandins. Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate, and reducing the second into a water molecule, with two electrons provided by NADPH via cytochrome P450 reductase (CPR; NADPH-ferrihemoprotein reductase).
• Molecular Mass: 59 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Concentration: >50 μg/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: Cyp4x1 cytochrome P450, family 4, subfamily x, polypeptide 1 [ Mus musculus (house mouse) ]
• Official Symbol: Cyp4x1
• Synonyms: CYP4X1; cytochrome P450, family 4, subfamily x, polypeptide 1; cytochrome P450, 4x1; CYP_a; A230025G20; Cyp4a28-ps
• Gene ID: 81906
• mRNA Refseq: NM_001003947
• Protein Refseq: NP_001003947
• UniProt ID: Q6A152