Recombinant Mouse Cyp2r1 Protein, Myc/DDK-tagged

• Cat.No.: Cyp2r1-2418M
• Product Overview: Purified recombinant protein of mouse full-length cytochrome P450, family 2, subfamily r, polypeptide 1 (Cyp2r1), with C-terminal MYC/DDK tag, expressed in HEK293T cells.

Specification

• Description: A cytochrome P450 monooxygenase involved in activation of vitamin D precursors. Catalyzes hydroxylation at C-25 of both forms of vitamin D, vitamin D2 and D3 (calciol). Can metabolize vitamin D analogs/prodrugs 1alpha-hydroxyvitamin D2 (doxercalciferol) and 1alpha-hydroxyvitamin D3 (alfacalcidol) forming 25-hydroxy derivatives. Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate, and reducing the second into a water molecule, with two electrons provided by NADPH via cytochrome P450 reductase (CPR; NADPH-ferrihemoprotein reductase).
• Source: HEK293T
• Species: Mouse
• Tag: Myc&DDK
• Molecular Mass: 57.3 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Concentration: >50 μg/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: Cyp2r1 cytochrome P450, family 2, subfamily r, polypeptide 1 [ Mus musculus (house mouse) ]
• Official Symbol: Cyp2r1
• Synonyms: Cyp2r1; cytochrome P450, family 2, subfamily r, polypeptide 1; vitamin D 25-hydroxylase; cytochrome P450, 2r1; EC 1.14.14.24
• Gene ID: 244209
• mRNA Refseq: NM_177382
• Protein Refseq: NP_796356
• UniProt ID: Q6VVW9