Recombinant Mouse Cyp11a1 Protein, Myc/DDK-tagged

• Cat.No.: Cyp11a1-2409M
• Product Overview: Purified recombinant protein of mouse full-length cytochrome P450, family 11, subfamily a, polypeptide 1 (Cyp11a1), with C-terminal MYC/DDK tag, expressed in HEK293T cells.

Specification

• Species: Mouse
• Source: HEK293
• Tag: DDK&Myc
• Description: A cytochrome P450 monooxygenase that catalyzes the side-chain hydroxylation and cleavage of cholesterol to pregnenolone, the precursor of most steroid hormones. Catalyzes three sequential oxidation reactions of cholesterol, namely the hydroxylation at C22 followed with the hydroxylation at C20 to yield 20R,22R-hydroxycholesterol that is further cleaved between C20 and C22 to yield the C21-steroid pregnenolone and 4-methylpentanal. Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate and reducing the second into a water molecule. Two electrons are provided by NADPH via a two-protein mitochondrial transfer system comprising flavoprotein FDXR (adrenodoxin/ferredoxin reductase) and nonheme iron-sulfur protein FDX1 or FDX2 (adrenodoxin/ferredoxin).
• Molecular Mass: 60.3 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Concentration: >50 μg/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: Cyp11a1 cytochrome P450, family 11, subfamily a, polypeptide 1 [ Mus musculus (house mouse) ]
• Official Symbol: Cyp11a1
• Synonyms: Cyp11a1; cytochrome P450, family 11, subfamily a, polypeptide 1; Sc; Scc; csc; Cyp1; cscc; P450s; Cyp11a; Cypxia1; P450scc; D9Ertd411; D9Ertd411e; cholesterol side-chain cleavage enzyme, mitochondrial; cholesterol desmolase; cytochrome P450 11A1; cytochrome P450 side-chain cleavage enzyme; cytochrome P450(scc); cytochrome P450, 11a, cholesterol side-chain cleavage; EC 1.14.15.6
• Gene ID: 13070
• mRNA Refseq: NM_019779
• Protein Refseq: NP_062753
• UniProt ID: Q9QZ82