Recombinant Mouse CST3 Protein, His-tagged

• Cat.No.: CST3-373M
• Product Overview: Recombinant Mouse CST3, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Specification

• Species: Mouse
• Source: Insect Cells
• Tag: His
• Description: CST3, also known as Cystatin-C is a member of family 2 of the cystatin superfamily. This protein is a secreted type 2 cysteine protease inhibitor synthesized in all nucleated cells, has been proposed as a replacement for serum creatinine for the assessment of renal function, particularly to detect small reductions in glomerular filtration rate. It is involved in processes such as tumor invasion and metastasis, inflammation and some neurological diseases. It inhibits many cysteine proteases such as papain and cathepsins B, H, K, L and S. It indicates that a murine model should be relevant for studies of the human disease, hereditary Cystatin C amyloid angiopathy.
• Form: Liquid. In Phosphate Buffered Saline (pH 7.4) containing 10% glycerol.
• Molecular Mass: 14.2kDa (126aa) confirmed by MALDI-TOF13.5-18KDa (SDS-PAGE under reducing conditions.)
• AA Sequence: ATPKQGPRML GAPEEADANE EGVRRALDFA VSEYNKGSND AYHSRAIQVV RARKQLVAGV NYFLDVEMGR TTCTKSQTNL TDCPFHDQPHLMRKALCSFQ IYSVPWKGTH SLTKFSCKNA HHHHHH
• Endotoxin: < 1.0 EU per 1 microgram of protein (determined by LAL method)
• Purity: >95% by SDS - PAGE
• Storage: Can be stored at +4 centigrade short term (1-2 weeks). For long term storage, aliquot and store at -20 centigrade or -70 centigrade. Avoid repeated freezing and thawing cycles.
• Concentration: 1mg/ml (determined by Absorbance at 280nm)

Gene Information

• Gene Name: Cst3 cystatin C [ Mus musculus ]
• Official Symbol: CST3
• Synonyms: CST3; cystatin C; cystatin-C; cystatin 3; cystatin-3; CysC
• Gene ID: 13010
• mRNA Refseq: NM_009976
• Protein Refseq: NP_034106
• Pathway: Salivary secretion, organism-specific biosystem; Salivary secretion, conserved biosystem
• Function: beta-amyloid binding; cysteine-type endopeptidase inhibitor activity; endopeptidase inhibitor activity; peptidase inhibitor activity; protease binding