Recombinant Mouse Cryaa Protein, His-tagged

• Cat.No.: Cryaa-851M
• Product Overview: Recombinant Mouse Cryaa Protein (Asp2-Ser173) with His tag was expressed in E. coli.

Specification

• Species: Mouse
• Source: E.coli
• Tag: His
• Protein Length: Asp2-Ser173
• Description: This gene encodes subunit a, one of two subunits of alpha-crystallin, which is a high molecular weight, soluble aggregate and is a member of the small heat shock protein (sHSP) family. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. It acts as a molecular chaperone and is the major protein in the eye lens, maintaining the transparency and refractive index of the lens. In mouse, deficiency in this gene is associated with smaller lenses and eyes and with increasing lens opacity with age. Alternative splicing results in multiple transcript variants.
• Form: Freeze-dried powder
• Molecular Mass: Predicted Molecular Mass: 23.7 kDa; Accurate Molecular Mass: 21 kDa
• Purity: > 90%
• Applications: Positive Control; Immunogen; SDS-PAGE; WB.
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 centigrade for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 centigrade for one month. Aliquot and store at -80 centigrade for 12 months.
• Storage Buffer: 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
• Reconstitution: Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Gene Information

• Gene Name: Cryaa crystallin, alpha A [ Mus musculus (house mouse) ]
• Official Symbol: Cryaa
• Synonyms: CRYAA; crystallin, alpha A; alpha-crystallin A chain; lens opacity 18; alpha-A-crystallin; crystallin, alpha 1; Crya1; lop18; Acry-1; Crya-1; DAcry-1; AI323437
• Gene ID: 12954
• mRNA Refseq: NM_013501
• Protein Refseq: NP_038529
• UniProt ID: Q569M7