Recombinant Mouse Crppa Protein, Myc/DDK-tagged

• Cat.No.: Crppa-1425M
• Product Overview: Purified recombinant protein of mouse full-length RIKEN cDNA 4930579E17 gene (cDNA clone MGC:106594 IMAGE:30610161), complete cds, with C-terminal MYC/DDK tag, expressed in HEK293T cells.

Specification

• Species: Mouse
• Source: HEK293
• Tag: Myc&DDK
• Description: Cytidylyltransferase required for protein O-linked mannosylation. Catalyzes the formation of CDP-ribitol nucleotide sugar from D-ribitol 5-phosphate. CDP-ribitol is a substrate of FKTN during the biosynthesis of the phosphorylated O-mannosyl trisaccharide (N-acetylgalactosamine-beta-3-N-acetylglucosamine-beta-4-(phosphate-6-)mannose), a carbohydrate structure present in alpha-dystroglycan (DAG1), which is required for binding laminin G-like domain-containing extracellular proteins with high affinity. Shows activity toward other pentose phosphate sugars and mediates formation of CDP-ribulose or CDP-ribose using CTP and ribulose-5-phosphate or ribose-5-phosphate, respectively. Not Involved in dolichol production.
• Molecular Mass: 31 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Concentration: >50 μg/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: Crppa CDP-L-ribitol pyrophosphorylase A [ Mus musculus (house mouse) ]
• Official Symbol: Crppa
• Synonyms: Crppa; CDP-L-ribitol pyrophosphorylase A; I; Ispd; AV040780; 4930579E17Rik; D-ribitol-5-phosphate cytidylyltransferase; 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase-like protein; isoprenoid synthase domain containing; isoprenoid synthase domain-containing protein; notch1-induced protein; EC 2.7.7.40
• Gene ID: 75847
• mRNA Refseq: NM_178629
• Protein Refseq: NP_848744
• UniProt ID: Q5RJG7