Recombinant Mouse Cox8c Protein, Myc/DDK-tagged

• Cat.No.: Cox8c-2280M
• Product Overview: Purified recombinant protein of mouse full-length cytochrome c oxidase subunit 8C (Cox8c), with C-terminal MYC/DDK tag, expressed in HEK293T cells.

Specification

• Description: Component of the cytochrome c oxidase, the last enzyme in the mitochondrial electron transport chain which drives oxidative phosphorylation. The respiratory chain contains 3 multisubunit complexes succinate dehydrogenase (complex II, CII), ubiquinol-cytochrome c oxidoreductase (cytochrome b-c1 complex, complex III, CIII) and cytochrome c oxidase (complex IV, CIV), that cooperate to transfer electrons derived from NADH and succinate to molecular oxygen, creating an electrochemical gradient over the inner membrane that drives transmembrane transport and the ATP synthase. Cytochrome c oxidase is the component of the respiratory chain that catalyzes the reduction of oxygen to water. Electrons originating from reduced cytochrome c in the intermembrane space (IMS) are transferred via the dinuclear copper A center (CU(A)) of subunit 2 and heme A of subunit 1 to the active site in subunit 1, a binuclear center (BNC) formed by heme A3 and copper B (CU(B)). The BNC reduces molecular oxygen to 2 water molecules using 4 electrons from cytochrome c in the IMS and 4 protons from the mitochondrial matrix.
• Molecular Mass: 8.5 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Concentration: >50 μg/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: Cox8c cytochrome c oxidase subunit 8C [ Mus musculus (house mouse) ]
• Official Symbol: Cox8c
• Synonyms: Cox8c; cytochrome c oxidase subunit 8C; COXV; COX8-; COX8-3; COXVIII-3; 1700007F21Rik; cytochrome c oxidase subunit 8C, mitochondrial; COX VIII-3; cytochrome c oxidase polypeptide 8; cytochrome c oxidase polypeptide VIII; cytochrome c oxidase subunit 8-3; cytochrome c oxidase subunit VIIIc
• Gene ID: 75483
• mRNA Refseq: NM_001039049
• Protein Refseq: NP_001034138
• UniProt ID: A6H666