Recombinant Mouse Cope Protein, Myc/DDK-tagged

• Cat.No.: Cope-2259M
• Product Overview: Purified recombinant protein of mouse full-length coatomer protein complex, subunit epsilon (Cope), with C-terminal MYC/DDK tag, expressed in HEK293T cells.

Specification

• Species: Mouse
• Source: HEK293
• Tag: DDK&Myc
• Description: The coatomer is a cytosolic protein complex that binds to dilysine motifs and reversibly associates with Golgi non-clathrin-coated vesicles, which further mediate biosynthetic protein transport from the ER, via the Golgi up to the trans Golgi network. The coatomer complex is required for budding from Golgi membranes, and is essential for the retrograde Golgi-to-ER transport of dilysine-tagged proteins. In mammals, the coatomer can only be recruited by membranes associated with ADP-ribosylation factors (ARFs), which are small GTP-binding proteins; the complex also influences the Golgi structural integrity, as well as the processing, activity, and endocytic recycling of LDL receptors.
• Molecular Mass: 34.6 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Concentration: >50 μg/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: Cope coatomer protein complex, subunit epsilon [ Mus musculus (house mouse) ]
• Official Symbol: Cope
• Synonyms: COPE; coatomer protein complex, subunit epsilon; coatomer subunit epsilon; epsilon-COP; epsilon-coat protein; nonclathrin coat protein epsilon-COP; Cope1; 1110005D17Rik
• Gene ID: 59042
• mRNA Refseq: NM_021538
• Protein Refseq: NP_067513
• UniProt ID: O89079