Recombinant Mouse CNTNAP2 cell lysate
Cat.No. : | CNTNAP2-2194MCL |
Product Overview : | Mouse CNTNAP2 / CASPR2 derived in Human Cells. The whole cell lysate is provided in 1X Sample Buffer.Browse all transfected cell lysate positive controls |
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Species : | Mouse |
Source : | Human Cells |
Tag : | Non |
Preparation method : | Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation and then centrifuged to clarify the lysate. The cell lysate was boiled for 5 minutes in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized. |
Lysis buffer : | Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF |
Quality control Testing : | 12.5% SDS-PAGE Stained with Coomassie Blue |
Recommended Usage : | 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200μL pure water and boiled for 2-5 min.3. Store it at -80°C. Recommend to aliquot the cell lysate into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.Notes:The lysate is ready to load on SDS-PAGE for Western blot application. If dissociating conditions are required, add reducing agent prior to heating. |
Stability : | Samples are stable for up to twelve months from date of receipt at -80°C |
Storage Buffer : | 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF |
Storage Instruction : | Lysate samples are stable for 12 months from date of receipt when stored at -80°C. Avoid repeated freeze-thaw cycles. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes. |
Gene Name | Cntnap2 contactin associated protein-like 2 [ Mus musculus ] |
Official Symbol | CNTNAP2 |
Synonyms | CNTNAP2; contactin associated protein-like 2; contactin-associated protein-like 2; cell recognition molecule Caspr2; Caspr2; mKIAA0868; 5430425M22Rik; |
Gene ID | 66797 |
mRNA Refseq | NM_001004357 |
Protein Refseq | NP_001004357 |
Pathway | Cell adhesion molecules (CAMs), organism-specific biosystem; Cell adhesion molecules (CAMs), conserved biosystem; |
Function | enzyme binding; protein binding; receptor binding; |
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CNTNAP2-4262HFL | Recombinant Full Length Human CNTNAP2 protein, Flag-tagged | +Inquiry |
◆ Cell & Tissue Lysates | ||
CNTNAP2-2194MCL | Recombinant Mouse CNTNAP2 cell lysate | +Inquiry |
Not For Human Consumption!
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