Recombinant Mouse Ciao1 Protein, Myc/DDK-tagged

• Cat.No.: Ciao1-2162M
• Product Overview: Purified recombinant protein of mouse full-length cytosolic iron-sulfur protein assembly 1 (Ciao1), with C-terminal MYC/DDK tag, expressed in HEK293T cells.

Specification

• Species: Mouse
• Source: HEK293
• Tag: DDK&Myc
• Description: Key component of the cytosolic iron-sulfur protein assembly (CIA) complex, a multiprotein complex that mediates the incorporation of iron-sulfur cluster into extramitochondrial Fe/S proteins. As a CIA complex component, interacts specifically with CIAO2A or CIAO2B and MMS19 to assist different branches of iron-sulfur protein assembly, depending of its interactors. The complex CIAO1:CIAO2B:MMS19 binds to and facilitates the assembly of most cytosolic-nuclear Fe/S proteins. CIAO1:CIAO2A specifically matures ACO1 and stabilizes IREB2. Seems to specifically modulate the transactivation activity of WT1. As part of the mitotic spindle-associated MMXD complex it may play a role in chromosome segregation.
• Molecular Mass: 37.6 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Concentration: >50 μg/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: Ciao1 cytosolic iron-sulfur protein assembly 1 [ Mus musculus (house mouse) ]
• Official Symbol: Ciao1
• Synonyms: CIAO1; cytosolic iron-sulfur protein assembly 1 homolog (S. cerevisiae); probable cytosolic iron-sulfur protein assembly protein CIAO1; WD40 protein Ciao1; WD repeat domain 39; WD repeat-containing protein 39; Wdr39; AW210570
• Gene ID: 26371
• mRNA Refseq: NM_025296
• Protein Refseq: NP_079572
• UniProt ID: Q99KN2