Recombinant Mouse Chst15 Protein, Myc/DDK-tagged

• Cat.No.: Chst15-2160M
• Product Overview: Purified recombinant protein of mouse full-length carbohydrate sulfotransferase 15 (Chst15), with C-terminal MYC/DDK tag, expressed in HEK293T cells.

Specification

• Species: Mouse
• Source: HEK293
• Tag: DDK&Myc
• Description: Sulfotransferase that transfers sulfate from 3'-phosphoadenosine 5'-phosphosulfate (PAPS) to the C-6 hydroxyl group of the GalNAc 4-sulfate residue of chondroitin sulfate A and forms chondroitin sulfate E containing GlcA-GalNAc(4,6-SO4) repeating units. It also transfers sulfate to a unique non-reducing terminal sequence, GalNAc(4SO4)-GlcA(2SO4)-GalNAc(6SO4), to yield a highly sulfated structure similar to the structure found in thrombomodulin chondroitin sulfate. May also act as a B-cell receptor involved in BCR ligation-mediated early activation that mediate regulatory signals key to B-cell development and/or regulation of B-cell-specific RAG expression; however such results are unclear in vivo.
• Molecular Mass: 65 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Concentration: >50 μg/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: Chst15 carbohydrate sulfotransferase 15 [ Mus musculus (house mouse) ]
• Official Symbol: Chst15
• Synonyms: CHST15; carbohydrate (N-acetylgalactosamine 4-sulfate 6-O) sulfotransferase 15; carbohydrate sulfotransferase 15; GalNAc4S-6ST; B-cell RAG-associated gene protein; GalNAc 4-sulfate 6-O-sulfotransferase; N-acetylgalactosamine 4-sulfate 6-O-sulfotransferase; BRAG; MAd5; mKIAA0598; GalNAcS-6ST; 4631426J05Rik
• Gene ID: 77590
• mRNA Refseq: NM_029935
• Protein Refseq: NP_084211
• UniProt ID: Q91XQ5