Recombinant Mouse Chmp1a Protein, Myc/DDK-tagged

• Cat.No.: Chmp1a-2145M
• Product Overview: Purified recombinant protein of mouse full-length charged multivesicular body protein 1A (Chmp1a), with C-terminal MYC/DDK tag, expressed in HEK293T cells.

Specification

• Description: Probable peripherally associated component of the endosomal sorting required for transport complex III (ESCRT-III) which is involved in multivesicular bodies (MVBs) formation and sorting of endosomal cargo proteins into MVBs. MVBs contain intraluminal vesicles (ILVs) that are generated by invagination and scission from the limiting membrane of the endosome and mostly are delivered to lysosomes enabling degradation of membrane proteins, such as stimulated growth factor receptors, lysosomal enzymes and lipids. The MVB pathway appears to require the sequential function of ESCRT-O, -I,-II and -III complexes. ESCRT-III proteins mostly dissociate from the invaginating membrane before the ILV is released. The ESCRT machinery also functions in topologically equivalent membrane fission events, such as the terminal stages of cytokinesis. ESCRT-III proteins are believed to mediate the necessary vesicle extrusion and/or membrane fission activities, possibly in conjunction with the AAA ATPase VPS4. Involved in cytokinesis. Involved in recruiting VPS4A and/or VPS4B to the midbody of dividing cells. May also be involved in chromosome condensation. Targets the Polycomb group (PcG) protein BMI1/PCGF4 to regions of condensed chromatin. May play a role in stable cell cycle progression and in PcG gene silencing.
• Source: HEK293T
• Species: Mouse
• Tag: Myc&DDK
• Molecular Mass: 21.6 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Concentration: >50 μg/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: Chmp1a charged multivesicular body protein 1A [ Mus musculus (house mouse) ]
• Official Symbol: Chmp1a
• Synonyms: CHMP1A; charged multivesicular body protein 1A; charged multivesicular body protein 1a; chromatin modifying protein 1A; chromatin-modifying protein 1a; procollagen (type III) N-endopeptidase; Pcoln3; 2900018H07Rik
• Gene ID: 234852
• mRNA Refseq: NM_145606
• Protein Refseq: NP_663581
• UniProt ID: Q921W0