Recombinant Mouse CD53 protein, His-tagged

• Cat.No.: CD53-2242M
• Product Overview: Recombinant Mouse CD53 protein(Q61451)(Glu107-Asn181), fused with His tag, was expressed in HEK293.

Specification

• Species: Mouse
• Source: HEK293
• Tag: His
• Protein Length: Glu107-Asn181
• Form: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Molecular Mass: The recombinant mouse CD53 consists of 95 amino acids and predicts a molecular mass of 10.6 KDa. It migrates as an approximately 22 KDa band in SDS-PAGE under reducing conditions due to glycosylation.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 90 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C
  Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: Cd53 CD53 antigen [ Mus musculus ]
• Official Symbol: CD53
• Synonyms: CD53; CD53 antigen; leukocyte surface antigen CD53; cell surface glycoprotein CD53; Ox-44; Tspan25; AI323659
• Gene ID: 12508
• mRNA Refseq: NM_007651
• Protein Refseq: NP_031677