Recombinant Mouse Cd40lg protein, monomeric hFc-tagged

• Cat.No.: Cd40lg-7854M
• Product Overview: Recombinant Mouse Cd40lg protein(P27548)(Met112-Leu260), fused with N-terminal monomeric hFc tag, was expressed in HEK293.

Specification

• Source: HEK293
• Species: Mouse
• Tag: N-monomeric hFc
• Protein length: Met112-Leu260
• Form: Lyophilized from 0.22μm filtered solution in PBS, 200mM L-arginine (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
• Bio-activity: Immobilized Mouse CD40, His Tag at 2 μg/ml (100 μl/well) on the plate. Dose response curve for Mouse CD40 Ligand Trimer, hFc Tag with the EC50 of 3.5 ng/ml determined by ELISA.
• Molecular Mass: The protein has a predicted MW of 75.6 kDa. Due to glycosylation, the protein migrates to 76-80 kDa based on Tris-Bis PAGE result.
• Endotoxin: Less than 1EU per μg by the LAL method.
• Purity: > 95% as determined by Tris-Bis PAGE
• Storage: Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
• Reconstitution: Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

Gene Information

• Gene Name: Cd40lg CD40 ligand [ Mus musculus ]
• Official Symbol: Cd40lg
• Synonyms: CD40LG; CD40 ligand; T-cell antigen Gp39; TNF-related activation protein; tumor necrosis factor ligand superfamily member 5; IGM; IMD3; Ly62; TRAP; gp39; CD154; Cd40l; HIGM1; Ly-62; T-BAM; CD40-L; Tnfsf5
• Gene ID: 21947
• mRNA Refseq: NM_011616
• Protein Refseq: NP_035746