Recombinant Mouse Cd28 Protein, His-tagged

• Cat.No.: Cd28-666M
• Product Overview: Recombinant Mouse Cd28 Protein (Asn20-Leu150) with His tag was expressed in E. coli.

Specification

• Species: Mouse
• Source: E.coli
• Tag: His
• Protein Length: Asn20-Leu150
• Description: Enables protein kinase binding activity. Acts upstream of or within several processes, including positive regulation of immune response; positive regulation of intracellular signal transduction; and regulation of lymphocyte activation. Located in external side of plasma membrane and immunological synapse. Human ortholog(s) of this gene implicated in multiple sclerosis and type 1 diabetes mellitus. Orthologous to human CD28 (CD28 molecule).
• Form: Freeze-dried powder
• Molecular Mass: Predicted Molecular Mass: 18.7 kDa; Accurate Molecular Mass: 19 kDa
• Purity: > 90%
• Applications: Positive Control; Immunogen; SDS-PAGE; WB.
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 centigrade for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 centigrade for one month. Aliquot and store at -80 centigrade for 12 months.
• Storage Buffer: 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
• Reconstitution: Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Gene Information

• Gene Name: Cd28 CD28 antigen [ Mus musculus (house mouse) ]
• Official Symbol: Cd28
• Synonyms: CD28; CD28 antigen; T-cell-specific surface glycoprotein CD28
• Gene ID: 12487
• mRNA Refseq: NM_007642
• Protein Refseq: NP_031668
• UniProt ID: P31041